Plasmid Preparation:Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions
Article Snippet: .. Following vectors were used: pSuper, pGW1-mCherry and pGW1-BFP, RTN4A-GFP (a gift from Dr. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61β (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherry myr 5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-ΔCoiled-coil, P180-Δrepeats-GFP, P180-Coiled-coil and P180-Repeats were previously described. ..
Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Article Snippet: .. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61b (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherrymyr5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-DCoiled-coil, P180-Drepeats-GFP, P180Coiled-coil and P180-Repeats were previously described.12 SplitAP-V5-C1 and 3xHA-split-EX-N1 were previously described.19 The plasmids generated in this study include: For GFP-LRRC59, LRRC59 sequence was PCR amplified from a cDNA library generated from a rat INS-1 cell line and inserted in pEGFP(A206K)-C1 vector between BglII and BamHI sites using HiFi DNA assembly (New England Biosciences). ..
Generated:Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Article Snippet: .. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61b (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherrymyr5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-DCoiled-coil, P180-Drepeats-GFP, P180Coiled-coil and P180-Repeats were previously described.12 SplitAP-V5-C1 and 3xHA-split-EX-N1 were previously described.19 The plasmids generated in this study include: For GFP-LRRC59, LRRC59 sequence was PCR amplified from a cDNA library generated from a rat INS-1 cell line and inserted in pEGFP(A206K)-C1 vector between BglII and BamHI sites using HiFi DNA assembly (New England Biosciences). ..
Sequencing:Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Article Snippet: .. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61b (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherrymyr5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-DCoiled-coil, P180-Drepeats-GFP, P180Coiled-coil and P180-Repeats were previously described.12 SplitAP-V5-C1 and 3xHA-split-EX-N1 were previously described.19 The plasmids generated in this study include: For GFP-LRRC59, LRRC59 sequence was PCR amplified from a cDNA library generated from a rat INS-1 cell line and inserted in pEGFP(A206K)-C1 vector between BglII and BamHI sites using HiFi DNA assembly (New England Biosciences). ..
Polymerase Chain Reaction:Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Article Snippet: .. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61b (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherrymyr5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-DCoiled-coil, P180-Drepeats-GFP, P180Coiled-coil and P180-Repeats were previously described.12 SplitAP-V5-C1 and 3xHA-split-EX-N1 were previously described.19 The plasmids generated in this study include: For GFP-LRRC59, LRRC59 sequence was PCR amplified from a cDNA library generated from a rat INS-1 cell line and inserted in pEGFP(A206K)-C1 vector between BglII and BamHI sites using HiFi DNA assembly (New England Biosciences). ..
Amplification:Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Article Snippet: .. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61b (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherrymyr5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-DCoiled-coil, P180-Drepeats-GFP, P180Coiled-coil and P180-Repeats were previously described.12 SplitAP-V5-C1 and 3xHA-split-EX-N1 were previously described.19 The plasmids generated in this study include: For GFP-LRRC59, LRRC59 sequence was PCR amplified from a cDNA library generated from a rat INS-1 cell line and inserted in pEGFP(A206K)-C1 vector between BglII and BamHI sites using HiFi DNA assembly (New England Biosciences). ..
cDNA Library Assay:Article Title: Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.
Article Snippet: .. Gia Voeltz, Addgene plasmid #61807), pEGFP(A206K)-N1 and pEGFP(A206K)-C1 (a gift from Dr. Jennifer Lippincott-Schwartz), GFP-Sec61b (a gift from Dr. Tom Rapoport, Addgene # 15108), RpL10A-tagRFP (a gift from dr. Robert Singer, Addgene #74172), TOM20-V5-FKBP-AP (a gift from Alice Ting, Addgene#120914), mCherrymyr5’/3’-Calreticulin (a gift from dr. Jefferey L. Twiss), Lifeact-mCherry (a gift from dr. Moritoshi Sato, Addgene #67302), HA-KifC1-MD-Strep, DP1-GFP, GFP-SBP-RTN4A, P180-DCoiled-coil, P180-Drepeats-GFP, P180Coiled-coil and P180-Repeats were previously described.12 SplitAP-V5-C1 and 3xHA-split-EX-N1 were previously described.19 The plasmids generated in this study include: For GFP-LRRC59, LRRC59 sequence was PCR amplified from a cDNA library generated from a rat INS-1 cell line and inserted in pEGFP(A206K)-C1 vector between BglII and BamHI sites using HiFi DNA assembly (New England Biosciences). ..
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